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![Ficoll 400 [Polysucrose 400], 5 Grams](https://m.media-amazon.com/images/I/61TIanDLQmL._SX466_.jpg)
Item #:
173919869
Ficoll 400 [Polysucrose 400], 5 Grams
Item #:
173919869
XAF 43408
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Ficoll is an uncharged polymer of sucrose widely used for stabilizing cells and isolation of nuclei and organelles.
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What Stands Out
High Purity
Ficoll 400 boasts exceptional purity, ensuring reliable results in laboratory applications. Its high-quality formulation minimizes contaminants, making it ideal for sensitive experiments.
Versatile Applications
Designed for various laboratory uses, Ficoll 400 effectively separates biological samples, including cells and proteins. Its adaptability addresses different research needs, enhancing experimental efficiency.
Convenient Packaging
Available in a convenient 5-gram package, Ficoll 400 simplifies usage and storage. This practical size reduces wastage, allowing researchers to maintain optimal workflow without compromising material integrity.
Product Details
| Item Form | Gel |
| Item Weight | 27.22 g |
| Brand Name | RPI |
| Manufacturer | RPI, Corp. |
| Part Number | F10400-5.0 |
| Brand | RPI |
Who Should Buy?
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Cell Separation
Ideal for researchers performing density gradient centrifugation to separate specific cell types in biological samples.
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Laboratory Use
Suitable for laboratories needing a reliable medium for isolating nucleated cells from blood or tissue samples.
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Biotechnology Applications
Beneficial for biotechnologists requiring a non-toxic, stable medium for various experimental procedures involving cell culture.
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Non-Research Use
Not suitable for casual or non-scientific users who lack the necessary expertise in handling this biochemical substance.
Product Description
Ficoll 400 [Polysucrose 400], 5 Grams
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Customer Questions & Answers
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Question:
What is Ficoll 400 [Polysucrose 400] used for?
Answer: Ficoll 400, also known as Polysucrose 400, is primarily utilized in laboratories for density gradient centrifugation. It is effective in isolating cells, such as lymphocytes, from whole blood or tissue samples. This makes it valuable in medical research, immunology studies, and cell culture applications. By creating a density gradient, Ficoll allows for the separation of different types of cells based on their buoyancy, ensuring high precision in cell isolation for experiments. -
Question:
How is Ficoll 400 prepared for use?
Answer: To prepare Ficoll 400, dissolve the powder in an appropriate buffer solution such as phosphate-buffered saline (PBS) to the desired concentration. It is important to gently mix the solution without creating bubbles, as air can affect the centrifugation process. Typical preparations involve creating a gradient by layering Ficoll over the sample. This method helps in obtaining pure cell populations and ensures optimal results in cellular assays. -
Question:
Can I use Ficoll 400 for isolating different cell types?
Answer: Yes, Ficoll 400 is versatile and can be used to isolate various cell types, including lymphocytes, monocytes, and even stem cells. Different cell types possess unique densities, making Ficoll an excellent choice for distinguishing and separating them during centrifugation. This capability is particularly advantageous in immunological research, cancer studies, and regenerative medicine, where understanding cell populations is crucial. -
Question:
Is Ficoll 400 toxic or hazardous?
Answer: Ficoll 400 is generally considered safe for laboratory use when handled properly. However, like many chemical products, it is essential to follow laboratory safety protocols and guidelines to minimize risks. Always use gloves and protective eyewear when working with Ficoll, and ensure that it is disposed of according to your institution's regulations. This precaution ensures a safe working environment while conducting experiments involving cell separation and analysis. -
Question:
What are common applications of Ficoll 400 in clinical research?
Answer: Ficoll 400 is widely used in clinical research for applications such as blood sample analysis, vaccine development, and hematopoietic system studies. Its capacity to isolate specific cell populations enables researchers to investigate immune responses, perform phenotyping, or conduct cellular assays more effectively. By facilitating the extraction of viable cells, Ficoll contributes to advancing therapies and understanding various medical conditions. -
Question:
How does Ficoll 400 compare to other cell separation techniques?
Answer: Ficoll 400 offers unique advantages over other cell separation techniques, such as magnetic bead separation or density-based centrifugation methods. It provides a gentle, non-toxic approach to isolate cells while maintaining their viability and functionality. Unlike other methods that may alter cell surface markers, Ficoll preserves the integrity of isolated cells, making it suitable for downstream applications like flow cytometry or functional assays. -
Question:
Can Ficoll 400 be stored, and if so, how?
Answer: Yes, Ficoll 400 can be stored, typically in a cool, dry place, away from direct sunlight. It is advisable to keep it sealed in its original container and to check for any signs of degradation before use. Proper storage ensures that Ficoll retains its efficacy for future laboratory applications, allowing researchers to maintain consistency in their experimental results over time. -
Question:
What precautions should I take when using Ficoll 400?
Answer: When using Ficoll 400, it is important to follow standard laboratory safety precautions, such as wearing gloves and protective eyewear. Additionally, ensure proper ventilation in your workspace and handle all samples with care to avoid contamination. Be mindful during centrifugation to avoid excessive force, which could lead to cell lysis. These practices help maintain a safe laboratory environment and ensure the reliability of experimental outcomes. -
Question:
How does Ficoll 400 affect cell viability post-separation?
Answer: Ficoll 400 is designed to maintain high cell viability post-separation, which is crucial for many downstream applications. Studies have shown that cells isolated using Ficoll demonstrate little to no damage, retaining their functionality for further assays. This makes it an ideal choice for applications in immunology, cellular therapy research, and any experiment requiring viable and functional cell populations. -
Question:
Where can I buy Ficoll 400 [Polysucrose 400], 5 Grams?
Answer: You can buy Ficoll 400 [Polysucrose 400], 5 Grams from Ubuy, a reliable e-commerce platform known for its diverse selection of laboratory supplies and reagents. Ubuy provides a convenient shopping experience, allowing you to easily browse and purchase the product you need for your research or experiments within Equatorial Guinea.
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XAF 43408
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Features & Benefits
- Ficoll is an uncharged polysucrose polymer.
- Ideal for stabilizing cells and isolating nuclei and organelles.
- Used in Denhardt's Reagent and gel loading buffers.
- Product sold for research or further manufacturing use only.
- Not for food or drug use.
- Molecular weight of 400,000 and CAS Number 26873-85-8.
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